How often should you inject TB-500?

TB-500 is typically injected twice weekly (2 mg per dose) during a 4–6 week loading phase, then once weekly (2 mg) for maintenance. This is much less frequent than BPC-157 (daily) because TB-500's longer half-life allows sustained tissue levels with weekly dosing.

TB-500 Injection Frequency: Loading and Maintenance Schedules

Why TB-500 Doses Weekly and BPC-157 Doses Daily

The two peptides have very different pharmacokinetic profiles. TB-500 (a synthetic fragment of Thymosin Beta-4) has a longer effective half-life in tissue, and its mechanism relies on sustained baseline levels rather than rapid pulses. BPC-157 has shorter residence time and appears to act more as an ongoing healing signal that benefits from daily reinforcement.

This difference has practical consequences: TB-500 tends to be more tolerable because injection burden is lower, but the per-dose amount is larger.

Standard Loading Phase (Weeks 1–4 or 1–6)

Dose: 2 mg subcutaneously, twice weekly
Schedule: Commonly split Monday/Thursday or Sunday/Wednesday — any ~3–4 day gap works
Rationale: higher initial dose saturates tissue and produces faster onset of systemic effects
Duration: 4 weeks for most injuries; extended to 6 weeks for severe or extensive tissue damage

Standard Maintenance Phase (Weeks 5+)

Dose: 2 mg subcutaneously once weekly
Schedule: Same day each week for consistency
Duration: 4–8 weeks typical, then evaluate

Alternative Dosing Schedules

Higher-Dose Loading for Severe Injuries

5 mg loading doses once weekly for 4 weeks
Used less commonly, requires careful clinician supervision
Higher cost per cycle

Lower-Dose Extended Maintenance

1 mg weekly for extended maintenance
Used in long-term protocols where acute healing has completed
Less commonly seen in practice

Injection Site and Technique

Subcutaneous injection in abdomen, thigh, or upper arm
29–31G insulin syringe
Rotate sites weekly
Avoid sites over active injuries during acute inflammation
Never inject TB-500 and BPC-157 in the same syringe

Reconstitution Notes

TB-500 arrives lyophilized and is reconstituted with bacteriostatic water per your compounding pharmacy's instructions. Common reconstitution:

5 mg vial reconstituted with 1 mL bacteriostatic water = 5 mg/mL solution
2 mg dose = 0.4 mL (40 units on 100-unit insulin syringe)
Reconstituted vial refrigerated, typically stable for 30 days

Timing Considerations

Time of day doesn't strongly matter — morning or evening
Can be administered with or without food
Don't inject within 24 hours of a competitive athletic event (both for WADA reasons and general recovery logic)

When to Deviate From Standard Schedule

Severe acute injury in a high-performing athlete — loading phase may be extended
Post-surgical recovery — typically starts 2 weeks post-op at maintenance dose
Patient intolerance of loading dose — reduce to 1 mg twice weekly during loading
Cost constraints — maintenance-only dosing is sometimes used for minor injuries

When to Stop

Full resolution of target injury
After 8–12 weeks with no meaningful response (unlikely to improve with longer cycle)
Any unusual systemic symptoms requiring evaluation
Before any cancer diagnosis workup
Before pregnancy attempts

See the TB-500 guide and Wolverine Stack dosage.